Phytochemical, antioxidant and anti-inflammatory analysis of dichloromethane extract of Archonea Cordifolia leaves

MO Bishop-Lawrence; OA Ohwokevwo; PC Ichipi-Ifukor; IO Okoro; FI Achuba

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Affiliations

MO Bishop-Lawrence
Department of Biochemistry, Faculty of Science, Delta State University, Abraka, Nigeria

OA Ohwokevwo
Department of Biochemistry, Faculty of Science, Delta State University of Science and Technology, Ozoro.

PC Ichipi-Ifukor
Department of Biochemistry, Faculty of Science, Delta State University, Abraka, Nigeria

IO Okoro
Department of Biochemistry, Faculty of Science, Delta State University, Abraka, Nigeria

FI Achuba
Department of Biochemistry, Faculty of Science, Delta State University, Abraka, Nigeria

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Bishop-Lawrence, M., Ohwokevwo, O., Ichipi-Ifukor, P., Okoro, I., & Achuba, F. (2025). Phytochemical, antioxidant and anti-inflammatory analysis of dichloromethane extract of Archonea Cordifolia leaves. International Journal of Forensic Medical Investigation, 11(1). Retrieved from https://ijfmi.org/index.php/JSD/article/view/213

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Phytochemical, antioxidant and anti-inflammatory analysis of dichloromethane extract of Archonea Cordifolia leaves

MO Bishop-Lawrence ,
OA Ohwokevwo ,
PC Ichipi-Ifukor ,
IO Okoro ,
FI Achuba

Vol 11 No 1 (2025): International Journal of Forensic Medical Investigation

Submitted: Jun 19, 2025

Published: May 5, 2025

Abstract

Background: Phytochemical constituents are integral to the therapeutic effectiveness of A. cordifolia.

Objectives: This study aims to thoroughly analyze the phytochemical, antioxidant, and anti-inflammatory properties of the dichloromethane extract of A. cordifolia leaves

Results: The percentage yield of the dichloromethane leaf extract was 0.92%, resulting in 6.42g of a brown mass. The extract's essential mineral composition highlights sodium and magnesium as the most prominent macro minerals, while iron and selenium are the most abundant micro minerals. The study revealed that tannins and phenols had the least concentration (0.17±0.02) among the phytochemicals, with alkaloids being the highest concentrated (11.7±0.06). The chromatogram identified various bioactive compounds in the extract, including alkanones, esters, fatty acids, alkanals, phenols, and aromatic compounds. The IC50 values of the extract were significantly (p<0.05) different compared to the respective standards. The in vitro anti-inflammatory response of the dichloromethane A. cordifolia leaf extract was assessed through inhibition of albumin denaturation, membrane stabilization, and anti-proteinase activities, all showing positive, concentration-dependent activities. Notably, the IC50 values for albumin denaturation and membrane stabilization were higher for the extract compared to aspirin. The dichloromethane extract of A. cordifolia leaves shows significant phytochemical, antioxidant, and anti- inflammatory properties, with high mineral content and potent bioactive compounds.

Conclusion: A. cordiforlia dichloromethane extract demonstrated strong in vitro antioxidant and anti-inflammatory activity, outperforming aspirin in albumin denaturation and membrane stabilization, indicating promising therapeutic potential

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Phytochemical, antioxidant and anti-inflammatory analysis of dichloromethane extract of Archonea Cordifolia leaves

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