2. Yamazaki H, Tsujimoto N, Koyanagi M, Katoh MC, Tajima K, Komori, M. Real-World Safety and Effectiveness of Tadalafil in Patients with Lower Urinary Tract Symptoms Secondary to Benign Prostatic Hyperplasia: A Japanese Post-Marketing Surveillance Study. Pragmatic and Observational Research. 2020; 11: 45-54.
3. Hatzimouratidis K, Salonia A, Adaikan G, Buvat J, Carrier S, El-Meliegy A, McCullough A, Torres LO, Khera M. Pharmacotherapy for Erectile Dysfunction: Recommendations From the Fourth International Consultation for Sexual Medicine (ICSM 2015). The Journal of sexual medicine. 2016; 13(4): 465–488.
4. Jin F, Gong QH, Xu YS, Wang LN, Jin H, Li F, Shi JS. Icariin, a phoshphodiesterase-5 inhibitor, improves learning and memory in APP/PS1 transgenic mice by stimulation of NO/cGMP signalling. International Journal of Neuropsychopharmacology, 2014; 17(6): 871-881.
5. Bischoff E. Potency, selectivity, and consequences of nonselectivity of PDE inhibition. International journal of impotence research. 2004; 16 (1): 11-14.
6. Noviasky J, Masood A, Vincent L. Tadalafil (Cialis) for erectile dysfunction. American family physician, 2004; 70: 359-60.
7. Carvajal JA, Germain AM, Huidobro-Toro JP, Weiner CP. Molecular mechanism of cGMP-mediated smooth muscle relaxation. Journal of Cellular Physiology. 2000; 184 (3): 409–420.
8. Shim YS, Pae CU, Cho KJ, Kim SW, Kim JC, Koh JS. Effects of daily low-dose treatment with phosphodiesterase type 5 inhibitor on cognition, depression, somatization and erectile function in patients with erectile dysfunction: a double-blind, placebo-controlled study. Int J Impot Res. 2014; 26:76–80.
9. García-Barroso C, Ricobaraza A, Pascual-Lucas M, Unceta N, Rico AJ, Goicolea MA, García-Osta A. Tadalafil crosses the blood–brain barrier and reverses cognitive dysfunction in a mouse model of AD. Neuropharmacology. 2013; 64: 114-123.
10. Baek SB, Bahn G, Moon SJ, Lee J, Kim KH, Ko IG, Shin MS. The phosphodiesterase type-5 inhibitor, tadalafil, improves depressive symptoms, ameliorates memory impairment, as well as suppresses apoptosis and enhances cell proliferation in the hippocampus of maternal-separated rat pups. Neuroscience letters. 2011; 488 (1): 26-30.
11. Zhang Q, Wu HH, Wang Y, Gu GJ, Zhang W, Xia R. Neural stem cell transplantation decreases neuroinflammation in a transgenic mouse model of Alzheimer's disease. Journal of Neurochemistry. 2016; 136 (4): 815-825.
12. Liebenberg N, Harvey BH, Brand L, Wegener G, Brink CB. Chronic treatment with the phosphodiesterase type 5 inhibitors sildenafil and tadalafil display anxiolytic effects in Flinders Sensitive Line rats. Metabolic brain disease, 2012; 27: 337-340.
13. Deng W, Aimone JB, Gage FH. New neurons and new memories: how does adult hippocampal neurogenesis affect learning and memory? Nature reviews neuroscience, 2010; 11 (5): 339-350.
14. Haxaire C, Turpin FR, Potier B, Kervern M, Sinet PM, Barbanel G, Billard JM. Reversal of age‐related oxidative stress prevents hippocampal synaptic plasticity deficits by protecting d‐serine‐dependent NMDA receptor activation. Aging cell. 2012; 11(2): 336-344.
15. Drury RAB, Wallington, EA. Preparation and fixation of tissues. Carlton’s Histological techniques. Oxford University press. 1980; 41-54.
16. Misra HP, Fridovich I. The role of superoxide anion in the autoxidation of epinephrine and a simple assay for superoxide dismutase. The Journal of biological chemistry. 1972; 247(10), 3170–3175.
17. Onavwose OP, Ichipi-Ifukor PC, Asagba SO. Single and Combined Aluminium and Cadmium Exposure during Pregnancy Mediate Changes in Cardio Metabolic Indices in Mice. Journal of Applied Sciences and Environmental. 2024; 28 (2):467-475
18. Irerhievwie O, Ichipi-Ifukor PC, Asagba SO. Hepatocellular degeneration in mice co-exposed to in-utero aluminium and cadmium: Implication of a disordered antioxidant and energy homeostatic response in the liver. Environmental Toxicology and Pharmacology, 2024; 106, 104375.
19. Cohen SN, Chang AC, Hsu L. Non chromosomal antibiotic resistance in bacteria: genetic transformation of Escherichia coli by R-factor DNA. Proceedings of the National Academy of Sciences of the United States of America. 1972; 69 (8): 2110–2114. https://doi.org/10.1073/pnas.69.8.2110
20. Ajayi AM, Ben-Azu B, Godson JC, Umukoro S. Effect of Spondias mombin fruit extract on scopolamine-induced memory impairment and oxidative stress in mice brain. J Herbs, Spices Med Plants. 2020; 27(1):24–36. https:// doi. org/ 10. 1080/ 10496 475. 2020. 17776 13
21. Daugan A, Grondin P, Ruault C, Le Monnier de Gouville AC, Coste H, Linget JM, et al. "The discovery of tadalafil: a novel and highly selective PDE5 inhibitor. 2:2,3,6,7,12,12a hexahydropyrazino [1',2':1,6] pyrido[3,4-b] indole-1,4-dione analogues". Journal of Medicinal Chemistry. 2003; 46 (21): 4533–4542
22. Reneerken OA, Rutten K, Akkerman S, Blockland A, Shaffer CL, Mennti F, et al. Phosphodiesterase type 5 (PDE5) inhibition improves object recognition memory; indications for central and peripheral mechanism. Neurobiology. 2012; 97: 370-379.
23. Allen DS, Wilson SK, Knapp PM. The efficacy and safety of Tadalafil in United States and Puerto Rican men with erectile dysfunction. J. Urol. 2004; 172: 652–7.
24. Robert J. Prefrontal cortex, in the encyclopedia of the human brain. 2002.
25. Hathaway WR, Newton BW. Neuroanatomy, Prefrontal Cortex. In: Stat Pearls. Treasure Island (FL): Stat Pearls Publishing; 2021
26. Nagdyman N, Fleck T, Bitterling B, Ewert P, Abdul-Khaliq H, Stiller B, et al. Influence of intravenous sildenafil on cerebral oxygenation measured by near-infrared spectroscopy in infants after cardiac surgery. Pediatr Res. 2006; 59:462–465.
- Abstract viewed - 677 times
- WORD downloaded - 163 times
License
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
Copyright
© PO Onyilo, EO Osakwe, CR Ejimeh, 2024
Affiliations
PO Onyilo
Department of Human Anatomy and Cell Biology, Faculty of Basic Medical Sciences, Delta State University, Abraka, Nigeria
EO Osakwe
Department of Human Anatomy and Cell Biology, Faculty of Basic Medical Sciences, Delta State University, Abraka, Nigeria
CR Ejimeh
Department of Human Anatomy and Cell Biology, Faculty of Basic Medical Sciences, Delta State University, Abraka, Nigeria
How to Cite
EFFECT OF TADALAFIL ON THE HIPPOCAMPUS AND PREFRONTAL CORTEX OF ADULT WISTAR RATS
Vol 10 No 1 (2024): Volume 10 Number 1
Submitted: Apr 11, 2024
Published: Apr 11, 2024
Abstract
Background: The hippocampus and prefrontal cortex works synergistically to encode consolidate and organize information into memory networks. This research aims to evaluate the histomorphological changes in the hippocampus and prefrontal cortex in adult Wistar rats following the administration of tadalafil, a medication used for the treatment of erectile dysfunction and benign prostate hypertrophy.
Materials and Methods: Fifteen adult Wistar rats, 130-180g in weight, were selected for the study and randomized into 3 groups. Group A, known as the control group, received only feed and water ad libitum. Groups B and C received 10 mg / kg and 20 mg / kg of tadalafil per body weight, respectively for 42 days. The hippocampus and prefrontal cortex of the rats were excised for histological and biochemical analysis. Data were analyzed using descriptive statistics, and the results were expressed as mean ± standard error of the mean. Differences in mean values were evaluated using one-way analysis of variance (ANOVA), with a significance level set at p<0.05.
Results: Tadalafil treatment did not alter the histomorphological features of the prefrontal cortex; however, there was evidence of increased blood flow. The oxidative stress assay revealed no significant changes in catalase (CAT) and acetyl cholinesterase (ACH) concentrations for treated rats compared to control. The hippocampal examination showed unique features such as giant pyramidal cells and the dentate gyrus (DG) with granular cells. The glia cells were sparsely distributed and the capillaries exhibited epithelial cells and the basal lamina. Hippocampal oxidative stress analysis indicated no significant alterations in superoxide dismutase (SOD) and glutathione peroxidase (GPX), but a decrease in CAT and ACH, coupled with an increase in MDA concentrations.
Conclusion: Tadalafil administration did not alter structure of hippocampus and prefrontal cortex in adult Wistar rat
Keywords: Tadalafil, Hippocampus, Prefrontal cortex, Rat